The protective effect of flagellin a and b as candidate vaccine against Pseudomonas aeruginosa respiratory infections

Background: Multi drug resistance (MDR) P. aeruginosa consider the main cause of morbidity in hospitalize patients suffering from chronic airway infections such as pulmonary disease, pneumonia and cystic fibrosis (CF). Also, rapidly development antibiotic to last generations a broad spectrum were detected for many isolates. Accordingly vaccines have potential prevent treatment infections. Aim: The present study aimed investigate active immunization strategies using flagellin order provide complete protection against MDR clinical isolates acute fatal respiratory infection animal model. Methods: Flagellin b purified partially mentioned previous study. After that animals immunized intranasally after sedative by applying 10 μl (4.8 μg), or μgl) on each nostril 100 mg weight rat at weekly intervals. week 6th dose, exposed 2*107 CFU directly into nostril.